Journal: Stem Cell Research & Therapy

Article Title: Development and validation of broad-spectrum magnetic particle labelling processes for cell therapy manufacturing

doi: 10.1186/s13287-018-0968-0

Figure Lengend Snippet: Microparticle-based labelling of adherent cultures incubated for 24 h in medium containing 1000 nm MPs (10 μg/μl). a – f Flow cytometry measurement of labelled (pink) and unlabelled (blue) cell populations analysed 24 h post incubation: a HeLa (cervical cancer stem cell line), b pMSC (primary mesenchymal stem cells), c HOS (human osteosarcoma stem cell line), d mESC (mouse embryonic stem cells), e SHSY5Y (human neuroblastoma derived stem cell line), f Caco-2 (human epithelial colorectal adenocarcinoma cells). Representative data shown, n = 3. g – i Fluorescence microscopy observation of neuroprogenitors (ReN, g ), mesenchymal stem cells (MSC, h ), and iPS-derived cardiomyocytes (CMC, i ) incubated overnight with MPs (gold), stained with Phalloidin (green) and Hoechst 33342 (blue)

Article Snippet: This paper describes labelling strategies using two MP size ranges (500 nm and 1000 nm) to label three therapeutically relevant stem cell populations: human bone marrow-derived MSC, iPS-derived human cardiomyocytes (CMC) and ReNeuron neural stem cells (ReN).

Techniques: Incubation, Flow Cytometry, Derivative Assay, Fluorescence, Microscopy, Staining

Journal: Stem Cell Research & Therapy

Article Title: Development and validation of broad-spectrum magnetic particle labelling processes for cell therapy manufacturing

doi: 10.1186/s13287-018-0968-0

Figure Lengend Snippet: Metabolic activity measurements of MSC ( a ), CMC ( b ) and ReN ( c ) cultures at 24 h post labelling using 500 nm and 1000 nm MPs at a range of concentrations ( a , b ) including the standard 10 μg/ml dose ( c ). Error bars presented as SEM, n = 8 ( a , b ) and n = 3 ( c ). * P ≤ 0.05, ** P ≤ 0.01, **** P ≤ 0.0001. MP magnetic particle

Article Snippet: This paper describes labelling strategies using two MP size ranges (500 nm and 1000 nm) to label three therapeutically relevant stem cell populations: human bone marrow-derived MSC, iPS-derived human cardiomyocytes (CMC) and ReNeuron neural stem cells (ReN).

Techniques: Activity Assay

Journal: Stem Cell Research & Therapy

Article Title: Development and validation of broad-spectrum magnetic particle labelling processes for cell therapy manufacturing

doi: 10.1186/s13287-018-0968-0

Figure Lengend Snippet: Development of suspension cell labelling approach for human cells. a , b MP-labelled MSC examined by fluorescence microscopy 24 h post labelling ( a ), after staining with Phalloidin (green) and Hoechst 33342 (blue), and by flow cytometry ( b ). c , d MSC viability examined by membrane integrity assay ( c ) and metabolic activity measurement ( d ) 24 h post labelling. Viable control consisted of unlabelled monolayer cells, Toxicity control consisted of cells exposed to 70% methanol for 10 min. e , f Fluorescence microscopy evaluation of CMC ( e ) and ReN ( f ) following suspension labelling with 500 nm (middle panel) or 1000 nm (right panel) MPs (gold) compared to unlabelled cells (left panel), with Phalloidin (green) and Hoechst 33342 (blue) counterstain. g , h Metabolic activity for CMC ( g ) and ReN ( h ) 24 h post suspension labelling. Error bars presented as SEM, n = 3. ** P ≤ 0.01. MP magnetic particle

Article Snippet: This paper describes labelling strategies using two MP size ranges (500 nm and 1000 nm) to label three therapeutically relevant stem cell populations: human bone marrow-derived MSC, iPS-derived human cardiomyocytes (CMC) and ReNeuron neural stem cells (ReN).

Techniques: Fluorescence, Microscopy, Staining, Flow Cytometry, Integrity Assay, Activity Assay

Journal: Stem Cell Research & Therapy

Article Title: Development and validation of broad-spectrum magnetic particle labelling processes for cell therapy manufacturing

doi: 10.1186/s13287-018-0968-0

Figure Lengend Snippet: Metabolic activity measurements of MP-labelled MSC ( a ), CMC ( b ) and ReN ( c ) after 24 h in HypoThermosol conditions. Error bars presented as SEM, n = 6

Article Snippet: This paper describes labelling strategies using two MP size ranges (500 nm and 1000 nm) to label three therapeutically relevant stem cell populations: human bone marrow-derived MSC, iPS-derived human cardiomyocytes (CMC) and ReNeuron neural stem cells (ReN).

Techniques: Activity Assay

Journal: Stem Cell Research & Therapy

Article Title: Development and validation of broad-spectrum magnetic particle labelling processes for cell therapy manufacturing

doi: 10.1186/s13287-018-0968-0

Figure Lengend Snippet: Assessment of labelled MSC and ReN after HypoThermosol storage for 24 h. a Differentiation of ReN analysed by immunodetection for MAP2, GFAP and β3-tubulin expression following a 7-day differentiation protocol. Additional examination of CMC forming beating cardiomyocyte clusters and analysis of alpha actinin expression in MP-labelled cell populations exposed to HypoThermosol storage shown in Additional file : Figure S4. b Osteogenic differentiation of MP-labelled MSCs for 14 days, post incubation in HypoThermosol analysed using Von Kossa staining to highlight mineral deposits formed after 14 days under induction treatment. Scale bar = 125 μm

Article Snippet: This paper describes labelling strategies using two MP size ranges (500 nm and 1000 nm) to label three therapeutically relevant stem cell populations: human bone marrow-derived MSC, iPS-derived human cardiomyocytes (CMC) and ReNeuron neural stem cells (ReN).

Techniques: Immunodetection, Expressing, Incubation, Staining